Early investigations revealed the driver was unable to avoid slamming into the giant mammal as it suddenly appeared from the forest.
The March 7 in Biloxi, Miss. happened while the bus was carrying 48 people from Austin, Texas stalled on railroad tracks.
The vehicle struck a tourist bus in Germany, slightly injuring the driver.
The company could face civil violations realted to the Sept. 17 crash that killed the driver and three passengers.
State Police have not yet pinpointed the cause of the multi-vehicle crash, which claimed the lives of two people.
Five people died and at least 18 people were wounded, some of whom lost limbs in the fiery crash on Aug. 2.
The bus killed eight passengers and injured dozens of others.
The charter bus carrying about 50 passengers rolled over May 14 in Texas, killing eight people.
City leaders may mandate local tour buses have a driver and a guide on board after a deadly accident last September.
The two vehicles carrying military personnel collided in north Fort Worth on March 19.
The NHTSA says seat belts alone would reduce the risk of serious or fatal injuries in rollover crashes by 77%.
Figures show 29% of fatal motorcoach crashes result in rollover; 70% of passenger fatalities stem from ejections.
The NTSB calls for a list of safety improvements after investigating a fatal 2014 bus crash.
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